LOCUS       sequence-75371-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, 
            cloDF13/spectinomycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-SPcas
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cacgttcagc tttcgttaaa ttatcaaact tacgttgagt gattaacttg gcgtttagaa
       61 gttgtctcca atagtttttc atctttttga ctacttcttc acttggaacg ttatccgatt
      121 taccacgatt tttatcagaa cgcgttaaga ccttattgtc tattgaatcg tctttaagga
      181 aactttgtgg aacaatgtga tcgacatcat aatcacttaa acgattaata tctaattctt
      241 ggtccacata catgtctctt ccattttgga gataatagag atagagcttt tcattttgca
      301 attgagtatt ttcaacagga tgctctttaa gaatctgact tcctaattct ttgatacctt
      361 cttcgattcg tttcatacgc tctcgcgaat ttttctggcc cttttgagtt gtctgatttt
      421 cacgtgccat ttcaataacg atattttctg gcttatgccg ccccattact ttgaccaatt
      481 catcaacaac ttttacagtc tgtaaaatac cttttttaat agcagggcta ccagctaaat
      541 ttgcaatatg ttcatgtaaa ctatcgcctt gtccagacac ttgtgctttt tgaatgtctt
      601 ctttaaatgt caaactatca tcatggatca gctgcataaa attgcgattg gcaaaaccat
      661 ctgatttcaa aaaatctaat attgttttgc cagattgctt atccctaata ccattaatca
      721 attttcgaga caaacgtccc caaccagtat aacggcgacg tttaagctgt ttcatcacct
      781 tatcatcaaa gaggtgagca tatgttttaa gtctttcctc aatcatctcc ctatcttcaa
      841 ataaggtcaa tgttaaaaca atatcctcta agatatcttc attttcttca ttatccaaaa
      901 aatctttatc tt
//
