LOCUS       sequence-75372-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Auyeung VC, Ulitsky I, McGeary SE, Bartel DP
  TITLE     Beyond Secondary Structure: Primary-Sequence Determinants License 
            Pri-miRNA Hairpins for Processing.
  JOURNAL   Cell. 2013 Feb 14;152(4):844-58. doi: 10.1016/j.cell.2013.01.031.
  PUBMED    23415231
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.01"; journalName: "Cell"; date: "2013-02-14- 
            14"; volume: "152"; issue: "4"; pages: "844-58"
COMMENT     Sequence Label: pcDNA3.2 mir-1-1 reporter mir50-2
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    55..79
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(261..285)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             638..679
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(719..738)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 cgaaattaat acgactcact atagggagac ccaagctggc tagttaagct atcaacaagt
       61 ttgtacaaaa aagcaggctc tatttttact aattctgaat tccataattc tatattctgt
      121 attcctcccc gccggccgct gatatgtctg gtattcttgg gtttgaactt ccagcgttga
      181 acccgcatat tagacgtatc gacggccggc ggggcataat ggctggtaag tttggagaga
      241 ccgggggagt cccagagatg ccccagcttt cttgtacaaa gtggttgatc tagagggccc
      301 gcagtggggt cagcttctac cggggcggcg tcccggggtc ttggaactgc atgcagactg
      361 cctgcttggg aaacatactt ctttatatgc ccatatggac ctgctaagct atggaatgta
      421 aagaagtatg tatctcaggc cgggacctct ctcgccgcac tgaggggcac tccacaccac
      481 gggggccgcc gcggccgaca gccagccagg cggctgtggt gggggtgagg ctggggcggc
      541 aggctgcctg ggcgttggtg gacggtcgtg tccccgccag cattgttctg tcactttgcc
      601 tgtgtcagca ggtggaagtg ctcaccagac attcgaaggt aagcctatcc ctaaccctct
      661 cctcggtctc gattctacgc gtaccggtta gtaatgagtt taaacggggg aggctaactg
      721 aaacacggaa ggagacaata ccggaaggaa cccgcgctat gacggcaata aaaagacaga
      781 ataaa
//
