LOCUS       sequence-75373-e         965 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sgRNA and Cas9-Puro in Drosophila S2 cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 965)
  AUTHORS   Bassett AR, Tibbit C, Ponting CP, Liu JL
  TITLE     Mutagenesis and homologous recombination in Drosophila cell lines 
            using CRISPR/Cas9.
  JOURNAL   Biol Open. 2013 Dec 10. pii: bio.20137120v1. doi: 
            10.1242/bio.20137120.
  PUBMED    24326186
REFERENCE   2  (bases 1 to 965)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biol Open. 
            2013 Dec 10. pii: bio.20137120v1. doi: 10.1242/bio.20137120."
COMMENT     Sequence Label: pAc-sgRNA-Cas9
FEATURES             Location/Qualifiers
     source          1..965
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(43..442)
                     /label=dU6-2 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-2 
                     snRNA (Das et al., 1987)"
ORIGIN
        1 acttgctatt tctagctcta aaacggaaga gctcgctctt ccgaagtatt gaggaaaaca
       61 tacctatata aatgatcaac atcaggaaag agcagttgag aattataaga attggcaaat
      121 ggtcttaaga accctctgct taagattttc aaaatttcct ttagaatcaa agtgtcctat
      181 tgttcgtttg ttgaaaacac agttcgattt attgactata ataaattgat agttttaaat
      241 atagaggcac gactaagaga gtttggtttt gttttggtta gggaccaaaa aaagtataca
      301 taacgaataa aaaaggattt aagtgcaaat ggtaaaaaag tgtcgagttt ttcttgagtt
      361 gattgtgctg taatgagact ctgcattcgg catttgactc ggcttttcct actcgtgccg
      421 tatttcaggc tgcaagtcga acagatctcg ctgcctgtta tgtggcccac aaaccaagac
      481 acgttttatg gccattaaag ctggctgatc gtcgccaaac accaaataca taatgaatat
      541 gtacacattc gagaaagaag cgatcaaaga agcgtcttcg ggcggagtag gagaatgcgg
      601 aggagaagga gaacgagctg atctagtatc tctccacaat ccaatgccaa ctgaccaact
      661 ggccatattc ggagcaattt gaagccaatt tccatcgcct ggcgatcgct ccattcttgg
      721 ctatatgttt ttcaccgtta cccggggcca ttttcaaaga ctcgtcggca agataagatt
      781 gtgtcactcg ctgtctctct tcatttgtcg aagaatgctg aggaatttcg cgatgacgtc
      841 ggcgagtatt ttgaagaatg agaataattt gtatttatac gaaaatcagt tagtggaatt
      901 ttctacaaaa acatgttatc tatagataat tttgttgcaa aatatgttga ctatgacaaa
      961 gattg
//
