LOCUS       sequence-75375-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian bicistronic expression of human Sox2 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLP-hSox2-ires-gfp
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    267..300
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(395..414)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gggccaggtt tccgggccct cacattgcca aaagacggca atatggtgga aaataacata
       61 tagacaaacg cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg
      121 gagggagagg ggcggatcca cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      181 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      241 ataaatgctt caataatatt ggatctataa cttcgtataa tgtatgctat acgaagttat
      301 ggatctttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
      361 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
      421 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
      481 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
      541 ggaatttcgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
      601 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
      661 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
      721 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
      781 gagtttcacc agttttgatt taaacgt
//
