LOCUS       sequence-75382-e         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral Destination vector containing AttR1-AttR4 site. Use with 
            4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 
            plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pREG(R1-R4)   #BGV059
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(134..257)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             378..803
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
ORIGIN
        1 agagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc
       61 tggggaatat aaatgtcagg ctcccttata cacagccagt ctgcaggtcg acgatatcgg
      121 cgcgccacta gtccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt
      181 tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt ctcgttcaac
      241 ttttctatac aaagttgccc ggggttatca gtggtccagg ctctagtttt gactcaacaa
      301 tatcaccagc tgaagcctat agagtacgag ccatagataa aataaaagat tttatttagt
      361 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta
      421 acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg
      481 tcaggaacag atggaacagg gtcgacccta gagaaccatc agatgtttcc agggtgcccc
      541 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc
      601 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc
      661 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca
      721 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta
      781 cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg
      841 gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg tgatgac
//
