LOCUS       sequence-75423-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses wt Akt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, 
            Turk BE, Yaffe MB, Sabatini DM
  TITLE     mTORC1 phosphorylation sites encode their sensitivity to starvation 
            and rapamycin.
  JOURNAL   Science. 2013 Jul 26;341(6144):1236566. doi: 
            10.1126/science.1236566.
  PUBMED    23888043
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2013-07-26"; volume: "341(6144):1236566. doi"; pages: " 
            10.1126/science.1236566"
COMMENT     Sequence Label: pRK5 HA-GST-Presc-mAkt1 (wt)
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             33..56
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 atcctccaaa aggtggaggt ggtgggtcga gtctggaagt tctgttccag gggccctggt
       61 cgacaatgaa cgacgtagcc attgtgaagg agggctggct gcacaaacga ggggaatata
      121 ttaaaacctg gcggccacgc tacttcctcc tcaagaacga tggcaccttt attggctaca
      181 aggaacggcc tcaggatgtg gatcagcgag agtccccact caacaacttc tcagtggcac
      241 aatgccagct gatgaagaca gagcggccaa ggcccaacac ctttatcatc cgctgcctgc
      301 agtggaccac agtcattgag cgcaccttcc atgtggaaac gcctgaggag cgggaagaat
      361 gggccaccgc cattcagact gtggccgatg gactcaagag gcaggaagaa gagacgatgg
      421 acttccgatc aggctcaccc agtgacaact caggggctga agagatggag gtgtccctgg
      481 ccaagcccaa gcaccgtgtg accatgaacg agtttgagta cctgaaacta ctgggcaagg
      541 gcacctttgg gaaagtgatt ctggtgaaag agaaggccac aggccgctac tatgccatga
      601 agatcctcaa gaaggaggtc atcgtcgcca aggatgaggt tgcccacacg cttactgaga
      661 accgtgtcct gcagaactct aggcatccct tccttacggc cctcaagtac tcattccaga
      721 cccacgaccg cctctgcttt gtcatggagt atgccaacgg gggcgagctc ttcttccacc
      781 tgtctcgaga gcgcgtgttc tccgaggacc gggcccgctt ctatggtgcg gagattgtgt
      841 ctgccctgga ctacttgcac tccgagaaga acgtggtgta ccgggacctg aagctggaga
      901 acctcatgct ggacaaggac gggcacatca agataacgga cttcgggctg tgcaaggagg
      961 ggatcaagga tggtgccact atgaagacat tctgcggaac
//
