LOCUS       sequence-75425-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses wt Akt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, 
            Turk BE, Yaffe MB, Sabatini DM
  TITLE     mTORC1 phosphorylation sites encode their sensitivity to starvation 
            and rapamycin.
  JOURNAL   Science. 2013 Jul 26;341(6144):1236566. doi: 
            10.1126/science.1236566.
  PUBMED    23888043
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2013-07-26"; volume: "341(6144):1236566. doi"; pages: " 
            10.1126/science.1236566"
COMMENT     Sequence Label: pRK5 HA-GST-Presc-mAkt1 (wt)
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     70..86
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     70..86
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttataagctg caataaacaa gttgggccat ggcggccaag ctggggatcc ttacttactt
       61 agcggccgct ctagaactag tggatcctca ggctgtgcca ctggctgagt aggagaactg
      121 ggggaagtgc ggcctccgct cactgtccac acactccatg ctgtcatctt gatcaggcgg
      181 cgtgatggtg atcatctgag ctgtgaactc ctcatcgaaa tacctggtgt cagtctcaga
      241 ggtgacctgg ggcttgaaag gtgggctcag cttcttctca tacacatcct gccacacgat
      301 gttggcaaag aaccggtgct gcatgatctc cttggcatcc tcagagcccc caccgagcct
      361 ctgtgtaggg tccttcttga gcagcccgga gagcagggac ttggcctcag ggccgagtgt
      421 gcgcgggaag cggatctcct ccatgaggat cagctcgaac agcttctcgt ggtcctggtt
      481 gtagaagggc aggcggccac acatcatctc atacatgacc acgcccagcc cccaccagtc
      541 cactgcacgg ccgtagtcgt tgtcctccag cacctcaggg gccaggtact ccggcgttcc
      601 gcagaatgtc ttcatagtgg caccatcctt gatcccctcc ttgcacagcc cgaagtccgt
      661 tatcttgatg tgcccgtcct tgtccagcat gaggttctcc agcttcaggt cccggtacac
      721 cacgttcttc tcggagtgca agtagtccag ggcagacaca atctccgcac catagaagcg
      781 ggcccggtcc tcggagaaca cgcgctctcg agacaggtgg aagaagagct cgcccccgtt
      841 ggcatactcc atgacaaagc agaggcggtc gtgggtctgg aatgagtact tgagggccgt
      901 aaggaaggga tgcctagagt tctgcaggac acggttctca gtaagcgtgt gggcaacctc
      961 atccttggcg acgatgacct ccttcttgag gatcttc
//
