LOCUS       sequence-75426-e        1722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces lentivirus expressing human FUS with FLAG tag at its 
            N-terminus by co-transfection with psPAX2 and pMD2G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Nakaya T, Alexiou P, Maragkakis M, Chang A, Mourelatos Z
  TITLE     FUS regulates genes coding for RNA-binding proteins in neurons by 
            binding to their highly conserved introns.
  JOURNAL   RNA. 2013 Apr;19(4):498-509. doi: 10.1261/rna.037804.112. Epub 2013 
            Feb 6.
  PUBMED    23389473
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037804.112"; journalName: "RNA"; date: "2013-04"; 
            volume: "19"; issue: "4"; pages: "498-509"
COMMENT     Sequence Label: pSLIK-NFLAG-hFUS
FEATURES             Location/Qualifiers
     source          1..1722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..78
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             459..470
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ttawkraagc cccgggaccg atccagcctc cgcggccccg aactagtcac catggactac
       61 aaggacgacg atgacaaaat ggcctcaaac gattataccc aacaagcaac ccaaagctat
      121 ggggcctacc ccacccagcc cgggcagggc tattcccagc agagcagtca gccctacgga
      181 cagcagagtt acagtggtta tagccagtcc acggacactt caggctatgg ccagagcagc
      241 tattcttctt atggccagag ccagaacaca ggctatggaa ctcagtcaac tccccaggga
      301 tatggctcga ctggcggcta tggcagtagc cagagctccc aatcgtctta cgggcagcag
      361 tcctcctatc ctggctatgg ccagcagcca gctcccagca gcacctcggg aagttacggt
      421 agcagttctc agagcagcag ctatgggcag ccccagagtg ggagctacag ccagcagcct
      481 agctatggtg gacagcagca aagctatgga cagcagcaaa gctataatcc ccctcagggc
      541 tatggacagc agaaccagta caacagcagc agtggtggtg gaggtggagg tggaggtgga
      601 ggtaactatg gccaagatca atcctccatg agtagtggtg gtggcagtgg tggcggttat
      661 ggcaatcaag accagagtgg tggaggtggc agcggtggct atggacagca ggaccgtgga
      721 ggccgcggca ggggtggcag tggtggcggc ggcggcggcg gcggtggtgg ttacaaccgc
      781 agcagtggtg gctatgaacc cagaggtcgt ggaggtggcc gtggaggcag aggtggcatg
      841 ggcggaagtg accgtggtgg cttcaataaa tttggtggcc ctcgggacca aggatcacgt
      901 catgactccg aacaggataa ttcagacaac aacaccatct ttgtgcaagg cctgggtgag
      961 aatgttacaa ttgagtctgt ggctgattac ttcaagcaga ttggtattat taagacaaac
     1021 aagaaaacgg gacagcccat gattaatttg tacacagaca gggaaactgg caagctgaag
     1081 ggagaggcaa cggtctcttt tgatgaccca ccttcagcta aagcagctat tgactggttt
     1141 gatggtaaag aattctccgg aaatcctatc aaggtctcat ttgctactcg ccgggcagac
     1201 tttaatcggg gtggtggcaa tggtcgtgga ggccgagggc gaggaggacc catgggccgt
     1261 ggaggctatg gaggtggtgg cagtggtggt ggtggccgag gaggatttcc cagtggaggt
     1321 ggtggcggtg gaggacagca gcgagctggt gactggaagt gtcctaatcc cacctgtgag
     1381 aatatgaact tctcttggag gaatgaatgc aaccagtgta aggcccctaa accagatggc
     1441 ccaggagggg gaccaggtgg ctctcacatg gggggtaact acggggatga tcgtcgtggt
     1501 ggcagaggag gctatgatcg aggcggctac cggggccgcg gcggggaccg tggaggcttc
     1561 cgagggggcc ggggtggtgg ggacagaggt ggctttggcc ctggcaagat ggattccagg
     1621 ggtgagcaca gacaggatcg cagggagagg ccgtattaac aaaaactcat ctcagaagag
     1681 gatctrtasg cggccgcact cgagatatct agacccagct tg
//
