LOCUS       sequence-75430-e         713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and PGK1 promoter controlled expression cassettes with 
            phleomycin resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G1-Ph
FEATURES             Location/Qualifiers
     source          1..713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(169..192)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(226..632)
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
ORIGIN
        1 cataaatcat aagaaattcg cttatttaga agtgtcaaca acgtatctac caacgatttg
       61 acccttttcc atcttttcgt aaatttctgg caaggtagac aagccgacaa ccttgattgg
      121 agacttgacc aaacctctgg cgaagaattg ttaattaaga gctcagatct tatcgtcgtc
      181 atccttgtaa tccatcgata ctagtgcggc cgcttgtaat taaaacttag attagattgc
      241 tatgctttct ttctaatgag caagaagtaa aaaaagttgt aatagaacaa gaaaaatgaa
      301 actgaaactt gagaaattga agaccgttta ttaacttaaa tatcaatggg aggtcatcga
      361 aagagaaaaa aatcaaaaaa aaaaattttc aagaaaaaga aacgtgataa aaatttttat
      421 tgcctttttc gacgaagaaa aagaaacgag gcggtctctt ttttcttttc caaaccttta
      481 gtacgggtaa ttaacgacac cctagaggaa gaaagagggg aaatttagta tgctgtgctt
      541 gggtgttttg aagtggtacg gcgatgcgcg gagtccgaga aaatctggaa gagtaaaaaa
      601 ggagtagaaa cattttgaag ctatggtgtg tgcggccggc ctggaagtac cttcaaagaa
      661 tggggtctta tcttgttttg caagtaccac tgagcaggat aataatagaa atg
//
