LOCUS       sequence-75431-e         720 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TEF1 and PGK1 promoter controlled expression cassettes with 
            phleomycin resistance for yeast transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Vickers CE, Bydder SF, Zhou Y, Nielsen LK
  TITLE     Dual gene expression cassette vectors with antibiotic selection 
            markers for engineering in Saccharomyces cerevisiae.
  JOURNAL   Microb Cell Fact. 2013 Oct 25;12(1):96.
  PUBMED    24161108
REFERENCE   2  (bases 1 to 720)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Microb Cell
            Fact."; date: "2013-10-25"; volume: "12(1)"; pages: "96"
COMMENT     Sequence Label: pCEV-G1-Ph
FEATURES             Location/Qualifiers
     source          1..720
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      24..189
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(79..103)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    complement(212..245)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(308..505)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 acgttgttga cacttctaaa taagcgaatt tcttatgatt tatgattttt attattaaat
       61 aagttataaa aaaaataagt gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa
      121 aattcttatt cttgagtaac tctttcctgt aggtcaggtt gctttctcag gtatagcatg
      181 aggtcgctcc tagtggatct gatatcacct aataacttcg tatagcatac attatacgaa
      241 gttatattaa gggttctcga gagctcgttt tcgacactgg atggcggcgt tagtatcgaa
      301 tcgacagcag tatagcgacc agcattcaca tacgattgac gcatgatatt actttctgcg
      361 cacttaactt cgcatctggg cagatgatgt cgaggcgaaa aaaaatataa atcacgctaa
      421 catttgatta aaatagaaca actacaatat aaaaaaacta tacaaatgac aagttcttga
      481 aaacaagaat ctttttattg tcagtactga tcatgagatg cctgcaagca attcgttctg
      541 tatcaggcgc aggagcgtcc cgtccgggtc gaccaaagcg gccatcgtgc ctccccactc
      601 ctgcagttcg ggggcatgga tgcgcggata gccgctgctg gtttcctgga tgccgacgga
      661 tttgcactgc cggtagaact ccgcgaggtc gtccagcctc aggcagcagc tgaaccaact
//
