LOCUS       sequence-75480-e        3091 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified pBluescript containing a telomeric insert..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Arat NO, Griffith JD
  TITLE     Human Rap1 interacts directly with telomeric DNA and regulates TRF2 
            localization at the telomere.
  JOURNAL   J Biol Chem. 2012 Dec 7;287(50):41583-94. doi: 
            10.1074/jbc.M112.415984. Epub 2012 Oct 20.
  PUBMED    23086976
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.415984"; journalName: "J Biol Chem"; date: 
            "2012-12-7- 7"; volume: "287"; issue: "50"; pages: "41583-94"
COMMENT     Sequence Label: f.pRST1
FEATURES             Location/Qualifiers
     source          1..3091
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(394..1185,1186..1254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1017..1036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1255..1359)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1385..1840)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1522..1543)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1734..1753
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1967..1989
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1981..1998
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1982..1998
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2008..2027
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2008..2026
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     2051..2067
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     2052..2068
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2232..2248)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2232..2248)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2285..2305)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2285..2303)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2324..2340)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2324..2340)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2337..2359)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2348..2364
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2372..2378,2379..2396,2397..2402))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2417..2438
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2555..2572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(2726..3091,1..223))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2806..2825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
       61 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      121 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      181 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      241 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      301 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      361 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      421 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      481 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
      541 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      601 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      661 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      721 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      781 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      841 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      901 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      961 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     1021 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1081 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1141 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1201 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1261 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1321 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1381 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     1441 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     1501 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     1561 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     1621 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     1681 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     1741 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     1801 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     1861 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     1921 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     1981 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc
     2041 gggccccccc tcgaggtcga cggtatcgat aagcttgaag acttagggtt agggttaggg
     2101 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     2161 ttagggttag ggttagggtt agggttaggg ttagggttag ggttagggtt agggttaggg
     2221 agacgaattc ggatccacta gttctagagc ggccgccacc gcggtggagc tccagctttt
     2281 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg
     2341 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     2401 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     2461 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     2521 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2581 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2641 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     2701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     3001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     3061 tatcgccact ggcagcagcc actggtaaca g
//
