LOCUS       sequence-75481-e         255 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplies the selection marker (blasticidin resistance) and IRES with
            flanked by AttR2 and AttL3 sites for Gateway cloning recombination 
            reaction. (This corresponds to a module 3 plasmid)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R2-iBlast-L3   #BGV204
FEATURES             Location/Qualifiers
     source          1..255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    55..71
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     79..95
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    148..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     172..188
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 accatgatta cgccactagc agatctgccg gccatagctg tttcctgtgt ggaattgtga
       61 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccact agcagatctg
      121 ccggccatag ctgtttcctg tgtggaattg tgagcggata acaatttcac acaggaaaca
      181 gctatgacca tgattacgcc actagcagat ctgccggcca tagctgtttc ctgtgtggaa
      241 ttgtgagcgg ataac
//
