LOCUS       sequence-75482-e         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses wt S6K1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Kang SA, Pacold ME, Cervantes CL, Lim D, Lou HJ, Ottina K, Gray NS, 
            Turk BE, Yaffe MB, Sabatini DM
  TITLE     mTORC1 phosphorylation sites encode their sensitivity to starvation 
            and rapamycin.
  JOURNAL   Science. 2013 Jul 26;341(6144):1236566. doi: 
            10.1126/science.1236566.
  PUBMED    23888043
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2013-07-26"; volume: "341(6144):1236566. doi"; pages: " 
            10.1126/science.1236566"
COMMENT     Sequence Label: pLJM60-S6K1(wt) barcoded
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     13..37
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        247..265
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     247..264
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     278..297
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     CDS             356..379
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc atccacgctg
       61 ttttgacctc catagaagac accgggaccg atccagcctc cgcggccggg aacggtgcat
      121 tggaacgcgg attccccgtg ccaagagtga cgtaagtacc gcctatagag tctataggcc
      181 cacccccttg gcttcgttag aacgcggcta caattaatac ataaccttat gtatcataca
      241 catacgattt aggtgacact atagaataac atccactttg cctttctctc cacaggtgtc
      301 cactcccagg tccaactgca cctcggttct atcgattgaa ttcaccatgg aaatggacta
      361 caaggatgac gatgacaagg gtggtggtgg tggtgcgtcg acgggatcca tggcaggagt
      421 gtttgacata gacctggacc agccagagga tgcaggctct gaggatgagc tggaggaggg
      481 gggtcagtta aatgaaagca tggaccatgg gggagttgga ccatatgaac ttggcatgga
      541 acattgtgag aaatttgaaa tctcagaaac tagtgtgaac agagggccag aaaaaatcag
      601 accagaatgt tttgagctac ttcgggtact tggtaaaggg ggctatggaa aggtttttca
      661 agtacgaaaa gtaacaggag caaatactgg gaagatattt gccatgaagg tgcttaaaaa
      721 ggcaatgata gtaagaaatg ctaaagatac agctcataca aaagcagagc ggaatattct
      781 ggaggaagta aagcatccct tcattgtgga tttaatttat gcctttcaga ccggtggaaa
      841 actctacctc atccttgagt atctcagtgg aggagaacta tttatgcagt tagaaagaga
      901 ggggatattc atggaagata cagcttgctt ttacttggct gaaatctcca tggctttggg
      961 gcattta
//
