LOCUS       sequence-75486-e         165 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Supplies the selection marker (puromycin resistance) and IRES with 
            flanked by AttR2 and AttL3 sites for Gateway cloning recombination 
            reaction. (This corresponds to a module 3 plasmid)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 165)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pBEG R2-iPuro-L3   #BGV205
FEATURES             Location/Qualifiers
     source          1..165
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..145)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccactagcag atctgccggc catagtgact ggatatgttg tgttttacag tattatgtag
       61 tctgtttttt atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc
      121 gttcagcttt cttgtacaaa gtggttcgat aattccgccc ccccc
//
