LOCUS       sequence-75492-e         873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Hartmann C, Tabin CJ
  TITLE     Dual roles of Wnt signaling during chondrogenesis in the chicken 
            limb.
  JOURNAL   Development. 2000 Jul . 127(14):3141-59.
  PUBMED    10862751
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2000 Jul . 127(14):3141-59."
COMMENT     Sequence Label: pGEM cBspII (CT#624)
FEATURES             Location/Qualifiers
     source          1..873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2..11
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(641..659)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(642..659)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(677..693)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(677..693)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(690..712)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    701..717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(725..731,732..749,750..755))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    770..791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gccatggcgg ccgcgggaat ttcgattatg caggtactca ggcagcaggc gaggggctga
       61 ccctcgggga tgtggggccg ggaggtgacg ctgcgtctgc ccatcaggac tgcaaagggg
      121 gccagaaggg cacacggggt gactcgggcg atgaggacag tgatgaggaa gaggaggaag
      181 aggaggagga ggaggaggaa gaagaggtag aggagcagga tgtcagtgtc aatgggacca
      241 gcaccaacac cacagcagag acaccccatg ggaacaacac cgtggcagct gaggaggagg
      301 aggatgatga tgaggaggag gaagaggaag aagaggagga ggaggaagct gaagccacca
      361 ccgctgctgc caccactgcg caggatgagg tgaccacgtt gggcgatgag cagcgctctg
      421 aggtcactac agctggggag cagtgggagt acgaggtgac agtgggagcc cgcggggatg
      481 aaggtcccac tgagagcagc tatggggacc aggaggagcc agcacgtggg gacagctacc
      541 gcgcctatga ggatgagtac aatcactagt gaattcgcgg ccgcctgcag gtcgaccata
      601 tgggagagct cccaacgcgt tggatgcata gcttgagtat tctatagtgt cacctaaata
      661 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      721 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      781 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      841 agctgcatta atgaatcggc caacgcgcgg ggg
//
