LOCUS       sequence-75498-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination dual luciferase reporter plasmid for checking 
            effectiveness of shRNA. Compatible with standard AttL1-AttL2 Gateway
            Entry vectors..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pCheck2 Dest (R1-R2)   #BGV129
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(111..235)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    251..299
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac catagtgact
      121 ggatatgttg tgttttacag tattatgtag tctgtttttt atgcaaaatc taatttaata
      181 tattgatatt tatatcattt tacgtttctc gttcagcttt cttgtacaaa gtggtgcggc
      241 cgctggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
      301 ggatctaaat gagtcttcgg acctcgcggg ggccgcttaa gcggtggtta gggtttgtct
      361 gacgcggggg gagggggaag gaacgaaaca ctctcattcg gaggcggctc ggggtttggt
      421 cttggtggcc acgggcacgc agaagagcgc cgcgatcctc ttaagcaccc ccccgccctc
      481 cgtggaggcg ggggtttggt cggcgggtgg taactggcgg gccgctgact cgggcgggtc
      541 gcgcgcccca gagtgtgacc ttttcggtct gctcgcagac ccccgggcgg cgccgccgcg
      601 gcggcgacgg gctcgctggg tcctaggctc catggggacc gtatacgtgg acaggctctg
      661 gagcatccgc acgactgcgg tgatattacc ggagaccttc tgcgggacga gccgggtcac
      721 gcggctgacg cggagcgtcc gttgggcgac aaacaccagg acggggcaca ggtacactat
      781 cttgtcaccc ggaggcgcga gggactgcag gagcttcagg gagtggcgca gctgcttcat
      841 ccccgtggcc cgttgctcgc gtttgctggc ggtgtccccg gaagaaatat atttgcatgt
      901 ctttagttct atgatgacac aaaccccgcc
//
