LOCUS       sequence-75502-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral Destination vector containing AttR1-AttR4 site. Use with 
            4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 
            plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4) .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pLEG(R1-R4)   #BGV057
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(132..255)
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    268..856
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(321..341)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(739..750)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac gatatcggcg
      121 cgccactagt ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
      181 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcaactt
      241 ttctatacaa agttgcccgg ggttaacaat caacctctgg attacaaaat ttgtgaaaga
      301 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
      361 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
      421 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
      481 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
      541 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
      601 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
      661 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
      721 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
      781 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
      841 tgggccgcct ccccgcctgg aattaattct gcagtcgaga cctagaaaaa catggagcaa
      901 tcacaagtag caatacagca gctacc
//
