LOCUS       sequence-75503-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral Destination vector containing AttR1-AttR3 site. For use 
            with three-way LR reaction with cDNA in entry vector and a marker in
            pBEG R2-L3 plasmids (module 2 and module 3 plasmids)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Geiling B, Vandal G, Posner AR, de Bruyns A, Dutchak KL, Garnett S, 
            Dankort D
  TITLE     A modular lentiviral and retroviral construction system to rapidly 
            generate vectors for gene expression and gene knockdown in vitro and
            in vivo.
  JOURNAL   PLoS One. 2013 Oct 11;8(10):e76279. doi: 
            10.1371/journal.pone.0076279.
  PUBMED    24146852
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-10-11"; volume: "8(10):e76279. doi"; pages: " 
            10.1371/journal.pone.0076279"
COMMENT     Sequence Label: pREG(R1-R3)   #BGV141
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(135..258)
                     /gene="mutant version of attR"
                     /label=attR3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     LTR             382..807
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(910..932)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcaggtcgac gatatcggcg
      121 cgccaacccc ggcccatagt gactggatat gttgtgtttt acagtattat gtagtctgtt
      181 ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt tctcgttcaa
      241 ctttattata catagttggc tagtggcgtt atcagtggtc caggctctag ttttgactca
      301 acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt
      361 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
      421 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc
      481 aaggtcagga acagatggaa cagggtcgac cctagagaac catcagatgt ttccagggtg
      541 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
      601 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
      661 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
      721 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
      781 actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc
      841 cagggaccac cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga
      901 cggtgaaaac ctctgacaca tgcagctccc gga
//
