LOCUS       sequence-75508-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  TITLE     Tet-On Advanced vectors
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLenti CMVtight Neo DEST (w770-1)
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(143..148,149..166,167..173)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(293..312)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tgtacaaaaa agctgaacga gaaacgtaaa atgatataaa tatcaatata ttaaattaga
       61 ttttgcataa aaaacagact acataatact gtaaaacaca acatatccag tcactatggc
      121 ggccgcatta ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat
      181 tttgagttag gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
      241 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
      301 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
      361 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
      421 cctgatgaat gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg
      481 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
      541 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
      601 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
      661 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
      721 cttcttcgcc
//
