LOCUS       sequence-75514-e         717 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overespression of Flp step-arrest mutant in yeast, LYS2 selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  TITLE     Gartenberg plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pMC6-TEFkFLP(LYS2)
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(3..21)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    62..565
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        602..707
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
       61 ggatcgcttg cctgtaactt acacgcgcct cgtatctttt aatgatggaa taatttggga
      121 atttactctg tgtttattta tttttatgtt ttgtatttgg attttagaaa gtaaataaag
      181 aaggtagaag agttacggaa tgaagaaaaa aaaataaaca aaggtttaaa aaatttcaac
      241 aaaaagcgta ctttacatat atatttatta gacaagaaaa gcagattaaa tagatataca
      301 ttcgattaac gataagtaaa atgtaaaatc acaggatttt cgtgtgtggt cttctacaca
      361 gacaagatga aacaattcgg cattaatacc tgagagcagg aagagcaaga taaaaggtag
      421 tatttgttgg cgatccccct agagtctttt acatcttcgg aaaacaaaaa ctattttttc
      481 tttaatttct ttttttactt tctattttta atttatatat ttatattaaa aaatttaaat
      541 tataattatt tttatagcac gtgatgaaaa ggacccaggt ggcacttttc ggggaaatgt
      601 gcgcggaacc cctatttgtt tatttttcta aatacattcn aatatgtatc cgctcatgag
      661 acaataaccn ctgataaatg cttcnataat attgaaaaag gaagagtatg agtattc
//
