LOCUS       sequence-75520-e        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-AmeR
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(113..140)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(156..227)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    946..965
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(971..977,978..993,994..999))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtagc
       61 tagcattata cctaggaagt gatgagttgt caagtttgtc actatctata aacgcagaaa
      121 ggcccacccg aaggtgagcc agtgtgactc tagtagagag cgttcaccga caaacaacag
      181 ataaaacgaa aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctgggggta
      241 tgggccctag ttattttgcc agcggattac gcagacctgc atcaatcaga tcaaccagct
      301 gatcacaacg ggttgccaga ttaccaatat cttcttgtgc aatcagaacc ggatgaataa
      361 cgctggtcag tgcatgcaga acggtttctg ccagaaccgg aatatcggtt gcaatataca
      421 gacctgcttc aacaccatca cgaataattt cggccagcag ttttgcaatc acgcttttgt
      481 aataatcgcc acatttcata tcctgtttgg cggtcatcag gatctgaata aaaacccgta
      541 tgtgtttgaa atgatcctga tgatgctgtt ccatcagatt acgtgccaga tgacgcagac
      601 gatccagcgg tgcatggctt ttatccagcg gacaaatctg acgttcaaaa acaccaatct
      661 gaccaaaacc aattgcatca accagtgcgt ttttgctgct aaaatgttta aacacatttg
      721 ccggactcat attcagtgcg cttgcaatat ctgcaatggc aactgcatta aaaccacgtt
      781 cacgaaacag ttcttcggct tttgccagaa tatcacgacg ggtttcttcg gcactacgac
      841 gcggacgacg acgaaccggc agatcgcttt tacgatcacc tttacgcacc tgatcaatgg
      901 ttttgttcat ctaatccccc tcgtatgtga aaacatagtc catagaattg tgagcgctca
      961 caattccaca cattatacga gccgatgatt aattgtcaac ac
//
