LOCUS       sequence-75524-e        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-BM3R1
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(131..158)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(174..245)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    873..892
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(898..904,905..920,921..926))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtagc
       61 tagcattatc ggaatgaacg ttcattccgt caattggtaa cgaatcagat tccaccgtac
      121 gtcgtataaa cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagagcg
      181 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
      241 atttgatgcc tgggggtatg ggccctagtt agctctgacg gctcagtgct gcccacaggc
      301 tttcttcaac accggtcagc agttcatcgg tcaggctcag ataatcgttc tcgatcattt
      361 catacacttc cataaagctg ccaaacagaa ttgcaatcag tgcattttcc ggcagattac
      421 gaatcacacc ctgtttctga ccttcacgaa aaaaggtgca cacaaattca accagtttct
      481 gatatgccag acggctttct tcggtcagaa aggtgccctg gctatgggtt ttgataaaac
      541 ccagtgcacg cggatgattt ttggtaaagg tcaccatacc ttcaaaaatg tgatgaaagc
      601 catcacgata accatcacgt tcatttgcca gaccgctttc aatacactgc agaaattcat
      661 taacatgctg ctgaaacagt tcgttcacca ggctttcttt gtttttgaaa tagcgataaa
      721 tggtgcctgc accaactttt gcattttctg caatcatcgg catggtggtt gcatcaaaac
      781 cacgttctgc aaacagcagc aggcttgcgc taaaaattgc tttctgtttg gtcggggtgc
      841 tttccatcta gtagttaaaa catagtccat agaattgtga gcgctcacaa ttccacacat
      901 tatacgagcc gatgattaat tgtcaacaca gccaacgatc gttccgatca gaacaagaaa
      961 gctatgtggc ctgacgaagc ggcgcgccat cgaatggcgc aaaac
//
