LOCUS       sequence-75527-e        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-ButR
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(155..182)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(198..269)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtagc
       61 tagcattata cctaggagtg acactgctgt caaagtgaca cgaatcagac aattgacggc
      121 tcgagggagt agcttccacc gtacgtcgta taaacgcaga aaggcccacc cgaaggtgag
      181 ccagtgtgac tctagtagag agcgttcacc gacaaacaac agataaaacg aaaggcccag
      241 tctttcgact gagcctttcg ttttatttga tgcctggggt atgggcccta gttagctgcg
      301 ttcacgcagt gctttttcaa tggtacgcat aacttcatcc agcggtgcat cggtacgtgc
      361 aacggtaacc agaacttcac cctgtgcgct aacggttgcc ggaccggctg caggcagggt
      421 atcacgacct gcaccaatac cggtgccaaa ggtgcgacga acaattgcaa atgcatgatc
      481 cagctgggtt tcaacatcac cctgaccacc ggcacgcagc caacgacgca gaacatgatt
      541 atgtgcggta acaactgcgc ttgctgcaac ttctgccagc agcggatcat cattgccatc
      601 atgatgtgca tgttcatcaa aatgacccag cagataacgg gtaaacagac gttcataacg
      661 tgcaacgctt gcaatttcac gttcacgcag ggtcggaact tcacgggtca gacgataacg
      721 ttcaacgcta actgccggac ttgctgcgta cattttcatc acttctttaa taccacgaca
      781 aacggtatcc agcggatgtt catgaggcgg tgctgcattc agaactgctt cggcacgaat
      841 cagggtatca tcatgatccg gaaaaatggc ttcttctttg ctacgaaaat gacgaaaaaa
      901 ggtacgacgt gcaacaccgg ctgctgctgc aatttcatca acggtggttg ctttataacc
      961 tttgcttgca aacagttcca ttgctgctgc tgccagttca g
//
