LOCUS       sequence-75529-e        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-HapR
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(131..158)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(174..245)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    914..933
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(939..945,946..961,962..967))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtatt
       61 atttgattaa aaatcaataa gagctagctg tcaattggta acgaatcaga caattgacgg
      121 ctcgtataaa cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagagcg
      181 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
      241 atttgatgcc tgggggtatg ggccctagtt agtttttata gatgcacagc atgttcagat
      301 agctatctgc cagtttataa actgcttctt gttcacccag gcgattaacc tgcagaaaga
      361 tgctataaaa gataccgtga aacaggctgg ccatattatc aacatcatgt ttttcgcaca
      421 gttcaccacg ttccattgct ttcataaaca tgttgcgaat cagcagctga ttggtacgat
      481 tggtgctaac aaacagaggc caaacttcat cacgggtgct tgctgaccat tcaaaccaaa
      541 ctttcagcca atgacaatcg gtcattgcca gtttaaccat ttctttgcaa acggtctgca
      601 gattggtttt aacatccaga tccagatcga tgtgatcggt cagaaaattg ctatactggc
      661 gaaccacaaa attcagaaca tcatcaacca gatcttcacg ggtcggaaaa tagttaaaca
      721 cggttgcaac gctaacctgt gcaatttctg caatatctgc atgaccacca cgaccaatac
      781 cacgttttgc aaaaacttcc agggcaattt ccatcagctg cagtttacgt ttctgcggac
      841 tcagacgggt acgcggacgt ttttcaatgc ttgcatccat ctagtatgtg tcctctttaa
      901 acatagtcca tagaattgtg agcgctcaca attccacaca ttatacgagc cgatgattaa
      961 ttgtcaacac agccaacgat cgttccgatc agaacaagaa agctatg
//
