LOCUS       sequence-75531-e        1002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-HlyIIR
FEATURES             Location/Qualifiers
     source          1..1002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             37..48
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(126..153)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(169..240)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    935..954
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(960..966,967..982,983..988))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ggtgaacagc tcctcgccct tgctcaccat ctagtatttc tcctctttct ctagtagcta
       61 gcattttaaa caagaatttt aaatatgtca attggtaacg aatcttccac cgtacgtcgt
      121 ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga gagcgttcac
      181 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
      241 atgcctgggg gtatgggccc tagttaaatg ttcggtttat cggtcagtgc gctaataata
      301 cggctaacca gatcttccgg catccattca tgattggtat cattggtttc attcggaccc
      361 aggctatcga tgaatttttt gaatttcgga aacagaacaa tgctggtaat ccaatggatg
      421 gtatggttga tgctaaaaaa gtgaaacaca ccctgttttt caccctcttg cagaatttct
      481 ttcagctgtt cgaagctgcc gataaaatac ggtttgattt tttccaggcg tgcgctttct
      541 ttgataattt cttcataggc cagggtgcca atttccggat tttctttaat gtgggtggta
      601 aaaacggtca gatattcacg cagggcatta atcggattaa actggttttt ttccagaaag
      661 ttcggcagtt catttgccag accgtatttt ttgaacacct cgtagtacag gttctctttg
      721 ccattaaagt aatagctggc cattgcaacg ttaactttgg cttctttggc aatttcttga
      781 atgctggtgc cttcataacc acgttcgccg aatttctttt tggctgcttt cagaatattt
      841 tccatggtct gttcacggct tttacccatt tcgcacacct caaacaggat gtatttcatt
      901 tagtgtttgt ccctctttca aacatagtcc atagaattgt gagcgctcac aattccacac
      961 attatacgag ccgatgatta attgtcaaca cagccaacga tc
//
