LOCUS       sequence-75538-e         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Basic backbone for transient transfection of Massively Parallel 
            Reporter Assay (MPRA) libraries.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Melnikov A, Zhang X, Rogov P, Wang L, Mikkelsen TS
  TITLE     Massively parallel reporter assays in cultured Mammalian cells.
  JOURNAL   J Vis Exp. 2014 Aug 17;(90). doi: 10.3791/51719.
  PUBMED    25177895
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Vis 
            Exp."; date: "2014-08-17"; volume: "(90). doi"; pages: " 
            10.3791/51719"
COMMENT     Sequence Label: pMPRA1
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    39..163
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(204..509)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(306..325)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gcggccggcc gccccgacta gcttggccgc cgaggccaac cactttgtac aagaaagctg
       61 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
      121 agactacata atactgtaaa acacaacata tccagtcact atggtcgacc tgcagactgg
      181 ctgtgtataa gggagcctga catttatatt ccccagaaca tcaggttaat ggcgtttttg
      241 atgtcatttt cgcggtggct gagatcagcc acttcttccc cgataacgga gaccggcaca
      301 ctggccatat cggtggtcat catgcgccag ctttcatccc cgatatgcac caccgggtaa
      361 agttcacggg agactttatc tgacagcaga cgtgcactgg ccagggggat caccatccgt
      421 cgcccgggcg tgtcaataat atcactctgt acatccacaa acagacgata acggctctct
      481 cttttatagg tgtaaacctt aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga
      541 gccgttcatt tcaataaacc gggcgacctc agccatccct tcctgatttt ccgctttcca
      601 gcgttcggca cgcagacgac gggcttcatt ctgcatggtt gtgcttacca gaccggagat
      661 attgacatca tatatgcctt gagcaactga tagctgtcgc tgtcaactgt cactgtaata
      721 cgctgcttca tagcatacct ctttttgaca tacttcgggt atacatatca gtatatattc
      781 ttataccgca
//
