LOCUS       sequence-75556-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for cGMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Bhargava Y, Hampden-Smith K, Chachlaki K, Wood KC, Vernon J, 
            Allerston CK, Batchelor AM, Garthwaite J
  TITLE     Improved genetically-encoded, FlincG-type fluorescent biosensors for
            neural cGMP imaging.
  JOURNAL   Front Mol Neurosci. 2013 Sep 24;6:26. doi: 10.3389/fnmol.2013.00026.
  PUBMED    24068983
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2013-09-24"; volume: "6:26. doi"; pages: " 
            10.3389/fnmol.2013.00026"
COMMENT     Sequence Label: pTriEx4-H6-FGAm (FlincG3)
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        14..123
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     CDS             144..161
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             171..215
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             231..248
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             267..281
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
ORIGIN
        1 ggagttaatc cgggaccttt aattcaaccc aacacaatat attatagtta aataagaatt
       61 attatcaaat catttgtata ttaattaaaa tactatactg taaattacat tttatttaca
      121 atcaaaggag atataccatg gcacaccatc accaccatca ctcttctggt aaagaaaccg
      181 ctgctgcgaa atttgaacgc cagcacatgg actcgccacc gccttctggt ctggtccccc
      241 ggggcagcgc aggttctggt acgattgatg acgacgacaa gatggtactc cgacaggcat
      301 tccggaagtt caccaaatcc gaaaggtcca aggatctcat aaaggaggcc atccttgaca
      361 atgactttat gaagaacttg gagctgtcac agatccaaga gattgtggat tgtatgtacc
      421 cagtggagta cggcaaagac agctgcatca tcaaagaagg agatgtgggg tcactggtgt
      481 atgtcatgga agatggtaag gttgaagtta caaaagaagg cgtgaagctg tgcacaatgg
      541 gtcctggtaa agtgtttgga gagttggcta tcctttacaa ctgtacccgg acggcgaccg
      601 tcaaaactct tgtaaatgtg aaactctggg ccattgatcg acaatgtttt cagacgataa
      661 tgatgaggac aggacttatc aagcataccg agtatatgga atttttaaaa agcgttccaa
      721 cattccagag ccttcctgaa gagatcctca gtaagcttgc tgacgtcctt gaagagaccc
      781 actatgaaaa tggggaatat atcatcaggc aaggtgcaag aggggacacc ttctttatca
      841 tcagtaaagg aaaggttaat gtcactcgtg aagactcgcc caatgaagac ccagtctttc
      901 tta
//
