LOCUS       sequence-75558-e         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for cGMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Bhargava Y, Hampden-Smith K, Chachlaki K, Wood KC, Vernon J, 
            Allerston CK, Batchelor AM, Garthwaite J
  TITLE     Improved genetically-encoded, FlincG-type fluorescent biosensors for
            neural cGMP imaging.
  JOURNAL   Front Mol Neurosci. 2013 Sep 24;6:26. doi: 10.3389/fnmol.2013.00026.
  PUBMED    24068983
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2013-09-24"; volume: "6:26. doi"; pages: " 
            10.3389/fnmol.2013.00026"
COMMENT     Sequence Label: pTriEx4-H6-FGAm (FlincG3)
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(59..82)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(89..121)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     primer_bind     315..334
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     574..595
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(701..722)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttggccacac cagccaccac cttctgatag gcagcctgca cctgaggtta atcacttagt
       61 gatggtgatg gtgatggtgg tgctcgagat cctcggggtc ttccggggcg agttctggct
      121 ggcttgcacg tgtatacagc tgtgcggccg cctatcgtcc gaaacctcca actccaaggg
      181 tgtcaatgat gttgaaatca gacaggttgt actccagctt gtgccccagg atgttgccgt
      241 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
      301 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
      361 tgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
      421 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
      481 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcac
      541 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
      601 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat gctccctccg gtaccgccct
      661 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
      721 tgtgatctct cttctcgttg gggtctttcg aaagtttgga ctgggtgctc aggtagtggt
      781 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtaggcga
      841 gctgcacgcc gccgtcctcg atgttgtggc ggatcttgaa gttcgccttg atgccgttct
      901 tctgcttgtc ggccttgata tagacgttct tc
//
