LOCUS       sequence-75564-e         949 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Fluorescent reporter for cGMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 949)
  AUTHORS   Bhargava Y, Hampden-Smith K, Chachlaki K, Wood KC, Vernon J, 
            Allerston CK, Batchelor AM, Garthwaite J
  TITLE     Improved genetically-encoded, FlincG-type fluorescent biosensors for
            neural cGMP imaging.
  JOURNAL   Front Mol Neurosci. 2013 Sep 24;6:26. doi: 10.3389/fnmol.2013.00026.
  PUBMED    24068983
REFERENCE   2  (bases 1 to 949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2013-09-24"; volume: "6:26. doi"; pages: " 
            10.3389/fnmol.2013.00026"
COMMENT     Sequence Label: pC1-FGB (FlincG2)
FEATURES             Location/Qualifiers
     source          1..949
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     475..494
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     734..755
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(861..882)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 acgttaacaa caacaattgc attcatttta tgtttcacgg ttcaggggga ggtgtgggag
       61 gttttttaaa gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcagttatct
      121 agatccggtg gatcccgggc ccgcggtacc gtcgattttc catggcagct gagaatattg
      181 taggagatct tctagaaaga tgtacaagaa agctgggtct agatatctcg agtgcggccg
      241 cgaattgtgg cgtttcttga gaatcttcat tgcaaaggtt ttggattctt cactttttaa
      301 ctggaccagt tctactcgtc cgaaacctcc aactccaagg gtgtcaatga tgttaaatca
      361 gacaggttgt actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc
      421 ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg
      481 tagttgccgt cgtccttgaa gaagatggtg cgctcctgga tgtagccttc gggcatggcg
      541 gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg
      601 taggtcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg
      661 aacttcaggg tcagcttgcc gtaggtggca tcgccctcac cctcgccgga cacgctgaac
      721 ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc
      781 tcctcgccct tgctcaccat gctccctccg gtaccgccct tgtacagctc gtccatgccg
      841 agagtgatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
      901 gggtctttcg aaagtttgga ctgggtgctc aggtagtggt tgtcgggca
//
