LOCUS       sequence-75565-e         280 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CAS9 nuclease in mammalian cells. Used to modify genome of
            mouse embroys.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 280)
  AUTHORS   Fujii W, Kawasaki K, Sugiura K, Naito K
  TITLE     Efficient generation of large-scale genome-modified mice using gRNA 
            and CAS9 endonuclease.
  JOURNAL   Nucleic Acids Res. 2013 Aug 30.
  PUBMED    23997119
REFERENCE   2  (bases 1 to 280)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Aug 30."
COMMENT     Sequence Label: pCAG-T3-hCAS-pA
FEATURES             Location/Qualifiers
     source          1..280
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     64..83
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 aaataaattt cctttattag ccagaagtca gatgctcaag gggcttcatg atgtccccat
       61 aatttttggc agagggaaaa agatctcagt ggtatttgtg agccagggca ttggccacac
      121 cagccaccac cttctgatag gcagcctgca cctgaggagt gaattccgta cacttgcatg
      181 ctacctcgag tttttttttt tttttttttt tttttttttt tttttttttt tttttttttt
      241 tttttttttt tttttttttt tttttttttt tttttttttt
//
