LOCUS       sequence-75567-e         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CAS9 nuclease in mammalian cells. Used to modify genome of
            mouse embroys.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Fujii W, Kawasaki K, Sugiura K, Naito K
  TITLE     Efficient generation of large-scale genome-modified mice using gRNA 
            and CAS9 endonuclease.
  JOURNAL   Nucleic Acids Res. 2013 Aug 30.
  PUBMED    23997119
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Aug 30."
COMMENT     Sequence Label: pCAG-T3-hCAS-pA
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        79..97
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             158..181
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             191..211
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca
       61 ttttggcaaa gaattcgaaa ttaaccctca ctaaagggaa caaaagctgg agctccaccg
      121 cggtggcggc cgctctagcc cgggcggatc caccatggat tacaaggatg acgacgataa
      181 gatcatggcc ccaaagaaga agcggaaggt cggtatccac ggagtcccag cagccgacaa
      241 gaagtactcc attgggctcg atatcggcac aaacagcgtc ggctgggccg tcattacgga
      301 cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc gccacagcat
      361 aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg aagccacgcg
      421 gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct gctacctgca
      481 ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata ggctggagga
      541 gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg gcaatatcgt
      601 ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga agaagcttgt
      661 a
//
