LOCUS       sequence-75573-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains 8xLexA-binding elements.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Fujita T, Fujii H
  TITLE     Direct identification of insulator components by insertional 
            chromatin immunoprecipitation.
  JOURNAL   PLoS One. 2011;6(10):e26109. doi: 10.1371/journal.pone.0026109. Epub
            2011 Oct 17.
  PUBMED    22043306
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(10):e26109. doi"; pages: " 
            10.1371/journal.pone.0026109. Epub 2011 Oct 17"
COMMENT     Sequence Label: 8xLexA-binding elements/pMD20
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(307..324)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(307..323)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(316..338)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(531..550)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     650..672
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(710..728)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        796..900
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctatagggga aagcttgcat gcctgcaggt cgactctaga ggatctacta gtcatatgga
       61 ttaatctaga ttctctatcg ataggtacct cgactgctgt atataaaacc agtggttata
      121 tgtacagtac tgctgtatat aaaaccagtg gttatatgta cagtacgcct cgactgctgt
      181 atataaaacc agtggttata tgtacagtac tgctgtatat aaaaccagtg gttatatgta
      241 cagtacgctc gagatccgct gaatagagtc gacacaatcg gatccccggg taccgagctc
      301 gaattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
      361 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
      421 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggccctgat gcggtatttt
      481 ctccttacgc atctgtgcgg tatttcacac cgcatatagt gcactctcag tacaatctgc
      541 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
      601 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
      661 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
      721 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
      781 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
      841 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
      901 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
      961 gtttttgctc acccagaaac
//
