LOCUS       sequence-75574-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Le TT, Harlepp S, Guet CC, Dittmar K, Emonet T, Pan T, Cluzel P
  TITLE     Real-time RNA profiling within a single bacterium.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Jun 28. 102(26):9160-4.
  PUBMED    15967986
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2005 Jun 28. 102(26):9160-4."
COMMENT     Sequence Label: pZE31ms2
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..83)
                     /codon_start=1
                     /product="enterokinase recognition and cleavage site"
                     /label=enterokinase site
                     /translation="DDDDK"
     CDS             complement(90..125)
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
     primer_bind     149..165
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     150..166
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_RNA        complement(211..229)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(250..268)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     primer_bind     complement(308..324)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(308..324)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     RBS             335..346
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(join(356..381,382..387,388..404,405..410,
                     411..429))
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    386..404
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    411..429
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(450..468)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        534..636
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     primer_bind     complement(703..722)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agattaatta attaagcggc cgcatcgatc gggccctgag gcctgcaggg atccaagctt
       61 gtcgacccct tgtcatcgtc atctccaccg ttgtgattat gattatgatt atgattatga
      121 ttatgaccca tgggtaccgg gccccccctc gaggtcgacg gtatcgataa gcttgatatc
      181 gaattccgat cctctagagt cgacctgcag acatgggtga tcctcatgtt ttctagagtc
      241 gacctgcaga catgggtgat cctcatgttt tctagagtcg acctgcaggc atgcaagctg
      301 cccgggggat ccactagttc tagagcgggg tacctttctc ctctttaatg aattcggtca
      361 gtgcgtcctg ctgatgtgct cagtatctct atcactgata gggatgtcaa tctctatcac
      421 tgatagggac tcgaggtgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      481 tgtcatgata ataatggttt cttagacgtc gatatctggc gaaaatgaga cgttgatcgg
      541 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt
      601 gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
      661 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
      721 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
      781 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
      841 gctcatccgg aatt
//
