LOCUS       sequence-75576-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFNLDD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Fujita T, Kitaura F, Fujii H
  TITLE     A Critical Role of the Thy28-MYH9 Axis in B Cell-Specific Expression
            of the Pax5 Gene in Chicken B Cells.
  JOURNAL   PLoS One. 2015 Jan 21;10(1):e0116579. doi: 
            10.1371/journal.pone.0116579. eCollection 2015.
  PUBMED    25607658
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-21"; volume: "10(1):e0116579. doi"; pages: " 
            10.1371/journal.pone.0116579. eCollection 2015"
COMMENT     Sequence Label: 3xFNLDD/pMXs-I2
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             90..155
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             192..212
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(225..476,477..833)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     768..789
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
ORIGIN
        1 tcctctagac tgccggatct agctagttaa ttaaggatcc cagtgtggtg gtacgggaat
       61 cgtttagtga accgtcagaa ttaaccatgg actacaaaga ccatgacggt gattataaag
      121 atcatgacat cgattacaag gatgacgatg acaagcttgc ggccgcgaat tcatcgatag
      181 atcgatcccc tccaaaaaag aagagaaagg tcgaacctga gggaatgaaa gcgttaacgg
      241 ccaggcaaca agaggtgttt gatctcatcc gtgatcacat cagccagaca ggtatgccgc
      301 cgacgcgtgc ggaaatcgcg cagcgtttgg ggttccgttc cccaaacgcg gctgaagaac
      361 atctgaaggc gctggcacgc aaaggcgtta ttgaaattgt ttccggcgca tcacgcggga
      421 ttcgtctgtt gcaggaagag gaagaagggt tgccgctggt aggtcgtgtg gctgccggtg
      481 aaccacttct ggcgcaacag catattgaag gtcattatca ggtcgatcct tccttattca
      541 agccgaatgc tgatttcctg ctgcgcgtca gcgggatgtc gatgaaagat atcggcatta
      601 tggatggtga cttgctggca gtgcataaaa ctcaggatgt acgtaacggt caggtcgttg
      661 tcgcacgtat tgatgacgag gttaccgtta agcgcctgaa aaaacagggc aataaagtcg
      721 aactgttgcc agaaaatagc gagtttaaac caattgtcgt agatcttcgt cagcagagct
      781 tcaccattga agggctggcg gttggggtta ttcgcaacgg cgactggctg tagtcgag
//
