LOCUS       sequence-75579-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 3xFNLDD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H
  TITLE     Identification of telomere-associated molecules by engineered 
            DNA-binding molecule-mediated chromatin immunoprecipitation 
            (enChIP).
  JOURNAL   Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038/srep03171.
  PUBMED    24201379
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2013-11-8"; volume: "3:3171. doi"; pages: " 
            10.1038/srep03171"
COMMENT     Sequence Label: 3xFNLDD/pMXs-puro
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             92..157
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             194..214
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(227..478,479..835)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     primer_bind     770..791
                     /label=LexA
                     /note="LexA DNA binding domain, forward primer"
ORIGIN
        1 atcctctaga ctgccggatc tagctagtta attaaggatc ccagtgtggt ggtacgggaa
       61 ttcgtttagt gaaccgtcag aattaaccat ggactacaaa gaccatgacg gtgattataa
      121 agatcatgac atcgattaca aggatgacga tgacaagctt gcggccgcga attcatcgat
      181 agatcgatcc cctccaaaaa agaagagaaa ggtcgaacct gagggaatga aagcgttaac
      241 ggccaggcaa caagaggtgt ttgatctcat ccgtgatcac atcagccaga caggtatgcc
      301 gccgacgcgt gcggaaatcg cgcagcgttt ggggttccgt tccccaaacg cggctgaaga
      361 acatctgaag gcgctggcac gcaaaggcgt tattgaaatt gtttccggcg catcacgcgg
      421 gattcgtctg ttgcaggaag aggaagaagg gttgccgctg gtaggtcgtg tggctgccgg
      481 tgaaccactt ctggcgcaac agcatattga aggtcattat caggtcgatc cttccttatt
      541 caagccgaat gctgatttcc tgctgcgcgt cagcgggatg tcgatgaaag atatcggcat
      601 tatggatggt gacttgctgg cagtgcataa aactcaggat gtacgtaacg gtcaggtcgt
      661 tgtcgcacgt attgatgacg aggttaccgt taagcgcctg aaaaaacagg gcaataaagt
      721 cgaactgttg ccagaaaata gcgagtttaa accaattgtc gtagatcttc gtcagcagag
      781 cttcaccatt gaagggctgg cggttggggt tattcgcaac ggcgactggc tgtagtcgaa
      841 attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata
      901 accgggcagg ccatgtctgc ccgtatttcg cgt
//
