LOCUS       sequence-75589-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-LitR
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(128..155)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(171..242)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    919..938
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(944..950,951..966,967..972))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtagc
       61 tagcattata ctgacaattt ataaatttgt caattggtaa cgaatcttcc accgtacgtc
      121 gtataaacgc agaaaggccc acccgaaggt gagccagtgt gactctagta gagagcgttc
      181 accgacaaac aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt
      241 tgatgcctgg gggtatgggc cctagttatt tgtagatgca cagcatattc agaaagcaat
      301 ttgcggtttc ttccatttct tcgctgctgc tattacgatt ggcctgaata aacacgctat
      361 agcaaatacc atgcagcatt ttggtcagat tttccggtgt atgatcattg cacacttcat
      421 tgcgttcaat accctcttca aacatggttt tgatcacctg attggtattg ctatgggtgc
      481 tcagaaacag aggccaaact tcatcacggg tgctggttga ccattcaaac caaactttaa
      541 tccatttgtt gccggtctga acgctatcaa taatgttcag cagcagggta ttcagattgc
      601 tacgaacatc cagatccagg ctaatgctgt tattgatgaa ctggtgaaac tcgttttcca
      661 ctttgttcag aacatcatca accagatctt cacgggtcgg aaaatagtta aacacggttg
      721 caacgctaac ctgtgcaatt tctgcaatat ctgcatgacc accacgacca ataccacgct
      781 ggctaaaaac ttcaatggca atatccagca gctgttcttt acgtttttcc ggactcagac
      841 gggtacgcgg acgtttctga atggtatcca tcgaggtttc tcctgtgtga aaaccctgtg
      901 tgaaaacata gtccatagaa ttgtgagcgc tcacaattcc acacattata cgagccgatg
      961 attaattgtc aacacagcca acgatcgttc cgatcagaac
//
