LOCUS       sequence-75591-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-LmrA
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(143..170)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(186..257)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    882..901
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(907..913,914..929,930..935))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtaaa
       61 atatagtgac tggtctatta tcttgattcg accaccagtt gtcaattggt aacgaatcag
      121 attccaccgt acgtcgtata aacgcagaaa ggcccacccg aaggtgagcc agtgtgactc
      181 tagtagagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga
      241 gcctttcgtt ttatttgatg cctgggggta tgggccctag ttaacgtttc agcagatccg
      301 gaatacagct gctaatatgc agcagcggtg tgctattttt tgcggtcagg ctcagcagaa
      361 taccaccttc aatcattgca ttaacaacgg tgcttgcttc ttttgcacgg ctttcgctac
      421 aaccggtctg acgcagtttt tcttcataca cgctggccca ttctttatat gcttcatgac
      481 atgcttcacg cagcggttcg cttttcaggc tggtttctgc tgccagcaga ccaaccggca
      541 gaccttcaat atcttcggta cagctaaact gacagctcag ttctttcaga aatgcctgaa
      601 taccttctgc cggatcggta catgcttcca tacaatcggc gattttctgg cggatatatt
      661 ctttcatttc gttcactgct tcaattgcca gctgttcttt accacccgga aaatgataat
      721 acaggctacc tttcggtgca ccgctttctt tgataatctg attcagaccg gtgccataat
      781 aaccctgcag ctgaaacaga cgggttgctg cgctcagaat tttttcacgg ctatcaccat
      841 agctcatcga ggcctttcct gtgtgaaaac atagtccata gaattgtgag cgctcacaat
      901 tccacacatt atacgagccg atgattaatt gtcaacacag cccaacgatc gttccgatca
      961 gaacaagaaa gctatgtggc ctgacgaagc ggcgcgcc
//
