LOCUS       sequence-75594-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-Orf2
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     RBS             125..136
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(231..258)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(274..345)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt gctagcaacc
       61 taactgaaca gcagttagtg tcaattggta acgaatcaga caattgacgg ctcaccatct
      121 agtatttctc ctctttctct agtagctagc aacctaactg aaccgtcagt tagtgtcaat
      181 tggtaacgaa tcagacaatt gacggctcgt tccaccgtac gtcgtataaa cgcagaaagg
      241 cccacccgaa ggtgagccag tgtgactcta gtagagagcg ttcaccgaca aacaacagat
      301 aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tgggggtatg
      361 ggccctagtt aatctgctgc cggtgctgcc agacatgcgc taacatgtgc aatcagtgca
      421 tcatgcatgg tctgtgcacc tgtacccgga tgacccggag cggttgccag aacctggctt
      481 gccagaccat caatcagtgc ggtcagggtc agtgctgcac catctgcatc aaccggacga
      541 aaaacaccct gaccaacacc acgacgaata acatctgcaa cggtgccacg ccactgacga
      601 taatattcaa catgcagctg accaacggtg gtgctacgtg ctgcttctgc ccacagatcc
      661 agccaaacac accactgacg acgctgctgt tcggtacgcg gtgtctgcag ttcaatcagc
      721 agacgcagct catcaccggc atgacgtgcg cctgcggttg catctgcacg acgacgggta
      781 tcttcatcca tacaccaacg aactgctgct tccagcagtt catcacgacc cggaaaatga
      841 taatgaattg ctgcggtgct ggtatgacat gctgctgcaa tatctgcaac acgaactgca
      901 tgaaaaccac gttctgcaat cagacgaacg gtttcacgaa caatctgcag cggacgacca
      961 ccttcggtgc ttgccggagg cagacgaggt gcgctcggc
//
