LOCUS       sequence-75596-e        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-PsrA
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(59..93)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
     terminator      complement(131..158)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(174..245)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtagc
       61 tagcattata cctaggactg agctagctgt caatcaaacg tttgttcctt tccaccgtac
      121 gtcgtataaa cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagagcg
      181 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
      241 atttgatgcc tgggggtatg ggccctagtt atgcttttgc ggtggttgcg ctggtgctgg
      301 ttttgctacg cggacgcagc tgtgctgctg ccattgcttc atcggtaaca ccgctatctg
      361 cacgcatacc tgctgccaga aacggaacca tcagacgcat aacctgttca atgctggtgt
      421 taataccaaa atcggtttct gcaattgcac gcagtgcttt aataccgctc atgctaaatg
      481 ctgctgcacc cagcataaaa tgaacacgcc aaaacagttc cagaggcgga acacgcggtg
      541 ctgcttcatt aaccagcagc atataacgac ggaacacttt accatacata tcttccagat
      601 aacgacgcag atgaccctgg ctctggctaa atgccagacc cagcagacgc ataaaaatgc
      661 tcagatcatt attgctacga ggctgaactg ccagtgcctg ttcaaccagc atttccagca
      721 gttcttccag gctcggtttc tgttccggac gtgcctgacg acgttccagt tcacgttcca
      781 ggcttgcaca aaacggaccc agaaaacggc taaaaactgc ctgaatcagt gcttttttgc
      841 tgccaaaatg ataattcact gctgccagat taacaccggc tttgctggta atcagacgca
      901 ggctggtttc tgcaaaacca cgttctgcaa acagctgttc tgctgcatcc agaatacgtt
      961 caacggtttc gctctgtgcc atctagtagt tcgtatcctc
//
