LOCUS       sequence-75598-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-QacR
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(142..169)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(185..256)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    884..903
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(909..915,916..931,932..937))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtata
       61 tagaccgacc gctcagtctc tatactaaag taggactgag ctagctgtca attggtaacg
      121 aatcagacaa ttgactataa acgcagaaag gcccacccga aggtgagcca gtgtgactct
      181 agtagagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
      241 cctttcgttt tatttgatgc ctggagaacg tcgcagtcct tatttgctca ggccattcag
      301 aaagatctgg ctgaatttgt tcatcagttt aatgcgttcg ttgatattct gctcatgggt
      361 aaaggtaaca atgccattaa cggcatttgc tgcaattttg ctcacggcat taacatcatt
      421 aatgcaccat tcgccgttca gattaccttc tttaaagatc acgtggtagg catcgatgta
      481 tttgttttcc agtttgttca ttttctcgtt gatgctgttg gttttatagt actcggtata
      541 aaactcgatg atggcattct gcagcggata gtaatattcg gtggtcaggc tcagttcatt
      601 atacagatag aatttctcgc ggttggtttt gcatttgatt tgttcttttt tccactgctc
      661 ttgccatttg ctttcttcga tgttcaggat ttccagaaac aggttctctt tggttttaaa
      721 gtgataatac agattgcctt tgctgctttc gctcagttta acaatttcac cggtggtggt
      781 tgcgttatag ccatttttga taaacagttc tttggcaacg cccagaattt tatctttcag
      841 gttcatctaa ccggtgtcct gtgtgaaaag ccaatggcat ggcaattgtg agcgctcaca
      901 attccacaca ttatacgagc cgatgattaa ttgtcaacac agccaacgat cgttccgatc
      961 agaacaagaa agctatgtgg cctgacgaag cggcgcgc
//
