LOCUS       sequence-75602-e        7715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Retroviral vector endoding for mouse CTCF fused to GFP .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Nakahashi H, Kwon KR, Resch W, Vian L, Dose M, Stavreva D, Hakim O, 
            Pruett N, Nelson S, Yamane A, Qian J, Dubois W, Welsh S, Phair RD, 
            Pugh BF, Lobanenkov V, Hager GL, Casellas R
  TITLE     A genome-wide map of CTCF multivalency redefines the CTCF code.
  JOURNAL   Cell Rep. 2013 May 30;3(5):1678-89. doi: 
            10.1016/j.celrep.2013.04.024. Epub 2013 May 23.
  PUBMED    23707059
REFERENCE   2  (bases 1 to 7715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2013.04.024"; journalName: "Cell Rep"; date: 
            "2013-05-30- 30"; volume: "3"; issue: "5"; pages: "1678-89"
COMMENT     Sequence Label: pMy-CTCF-GFP
FEATURES             Location/Qualifiers
     source          1..7715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             7..600
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             1090..1506
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1436..1458
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1474..1490
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1516..1890
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1828..1844
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      1921..1930
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4141..4143,4144..4146,4147..4860)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4141..4143,4144..4146,4147..4857)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4186..4207)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4447..4466)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4794..4815
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             5162..5753
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5819..5836)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5990..6578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6070..6089)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6742..7533,7534..7602))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7365..7384
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7603..7707)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgcataatg aaagacccca cctgtaggtt tggcaagcta gcttaagtaa cgccattttg
       61 caaggcatgg aaaaatacat aactgagaat agaaaagttc agatcaaggt caggaacaga
      121 tggaacagct gaatatgggc caaagcggat atctgtggta agcagttcct gccccggctc
      181 agggccaaga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag
      241 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca
      301 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc
      361 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg
      421 agctcaataa aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt
      481 cgcccgggta cccgtattcc caataaagcc tcttgctgtt tgcatccgaa tcgtggactc
      541 gctgatcctt gggagggtct cctcagattg attgactgcc cacctcgggg gtctttcatt
      601 tggaggttcc accgagattt ggagacccca gcccagggac caccgacccc cccgccggga
      661 ggtaagctgg ccagcggtcg tttcgtgtct gtctctgtct ttgtgcgtgt ttgtgccggc
      721 atctaatgtt tgcgcctgcg tctgtactag ttagctaact agctctgtat ctggcggacc
      781 cgtggtggaa ctgacgagtt cggaacaccc ggccgcaacc ctgggagacg tcccaggtcg
      841 ggggccgttt ttgtggcccg acctgagtcc aaaaagcccg atcgttttgg actctttggt
      901 gcacccccct tagaggaggg atatgtggtt ctggtaggag acgagaacct aaaacagttc
      961 ccgcctccgt ctgaattttt gctttcggtt tgggaccgaa gccgcgccgc gcgtcttgtc
     1021 tgctgcagca tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat
     1081 atgggcccgg gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat
     1141 gtcgagcgga tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc
     1201 tgctctgcag aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga
     1261 gacctcatca cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac
     1321 caggtcccct acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag
     1381 ccctttgtac accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt
     1441 gaacctcctc gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta
     1501 ggcgccccca tatggccata tgagatctta tatggggcac ccccgcccct tgtaaacttc
     1561 cctgaccctg acatgacaag agttactaac agcccctctc tccaagctca cttacaggct
     1621 ctctacttag tccagcacga agtctggaga cctctggcgg cagcctacca agaacaactg
     1681 gaccgaccgg tggtacctca cccttaccga gtcggcgaca cagtgtgggt ccgccgacac
     1741 cagactaaga acctagaacc tcgctggaaa ggaccttaca cagtcctgct gaccaccccc
     1801 accgccctca aagtagacgg catcgcagct tggatacacg ccgcccacgt gaaggctgcc
     1861 gaccccgggg gtggaccatc ctctagactg ccggatccca gtgtggtggt acgggaattc
     1921 gccaccatgg aaggtgaggc ggttgaagcc attgtggagg agtctgaaac tttcattaaa
     1981 ggaaaagaaa gaaagactta ccagagacgc cgggaagggg gccaggaaga agatgcttgc
     2041 cacctgcccc agaaccagac agatgggggt gaggtggtcc aggatgtcaa cagcagtgta
     2101 cagatggtaa tgatggaaca gctggatcct acccttctcc agatgaagac tgaagtcatg
     2161 gagggtacag tggctccgga agcagaggct gcagtggacg atacccagat cataaccttg
     2221 caggttgtaa atatggagga acagcccatt aacataggag agcttcagct tgtccaagta
     2281 cctgttcctg tgacggtacc tgttgctact acttcagtag aagaacttca gggggcttat
     2341 gagaatgaag tgtctaaaga gggccttgca gaaagtgaac cgatgatatg tcacacctta
     2401 cctttgcctg aaggatttca ggtggtgaaa gtgggggcca atggagaagt ggagacacta
     2461 gagcagggcg agcttcctcc tcaggaagac tctagctggc aaaaagaccc agactatcag
     2521 ccaccagcca aaaaaacaaa gaaaaccaaa aagagcaaac ttcgttacac agaagagggc
     2581 aaagacgtgg atgtgtctgt gtatgatttt gaggaagaac agcaggaagg actgctgtct
     2641 gaggttaatg cagagaaagt agttggtaat atgaagcctc cgaagccaac aaaaattaaa
     2701 aaaaaaggtg taaagaaaac attccagtgt gagctttgca gttacacatg tccccggcgt
     2761 tcaaatttgg atcgtcacat gaaaagccac actgatgaga gaccacacaa atgccatctg
     2821 tgtggcagag cattcagaac agtgaccctc ctgaggaatc atctgaacac acacacaggt
     2881 actcgtcctc acaagtgccc agactgcgat atggcctttg tgaccagtgg agaattggtg
     2941 cggcatcgtc gttataaaca cactcatgag aaaccattta agtgttccat gtgtgattat
     3001 gccagtgtag aagtcagcaa attaaaacga cacattcgct ctcatactgg agagcgcccg
     3061 ttccagtgca gtttgtgcag ttatgccagc agggacacat acaagctgaa aaggcatatg
     3121 agaacccatt caggggaaaa accttatgaa tgttatattt gtcacgctcg gtttacccag
     3181 agtggtacca tgaagatgca cattttacag aagcacacag aaaatgtggc caaatttcat
     3241 tgtccccatt gtgacactgt catagcccga aaaagtgatt tgggtgtcca cttgcgaaag
     3301 cagcattcct atattgaaca gggcaaaaaa tgtcgctact gtgatgctgt gtttcatgag
     3361 cgatatgctc tcatccagca tcaaaaatca cacaaaaatg agaagcgctt caagtgtgac
     3421 cagtgtgatt atgcttgtag acaggagcgg cacatgatca tgcacaagcg cactcacacg
     3481 ggggagaagc cttatgcctg cagccactgc gacaagacct tccgccagaa acagctcctc
     3541 gacatgcatt tcaagcgcta tcatgatccc aactttgtcc ctgctgcctt tgtctgttcc
     3601 aagtgtggga aaacattcac ccgccggaac acaatggcaa gacatgcaga taactgtgct
     3661 ggtccagatg gcgtagaggg ggaaaatgga ggggagacaa agaagagcaa acgaggaaga
     3721 aaaagaaaga tgcgatctaa aaaagaagac tcctctgaca gtgaagaaaa tgctgagccg
     3781 gatctcgatg acaatgagga ggaggaggag cctgctgtag aaattgaacc tgagccagag
     3841 cctcagcccc agcctccgcc tccacctcag cctgtggccc cggccccccc acctgccaag
     3901 aagagaaggg ggcgacctcc tggaagaacc aaccagccca aacagaacca gccaacagcc
     3961 atcattcagg tcgaagatca gaatacaggt gcaattgaga acattatagt tgaagtcaaa
     4021 aaggagccag atgccgagcc tgcggagggg gaagaagagg aggctcaggc agccaccaca
     4081 gacgccccca acggagacct cacgcctgag atgatcctca gcatgatgga ccggtccggc
     4141 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     4201 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
     4261 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     4321 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     4381 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     4441 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     4501 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     4561 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     4621 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     4681 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     4741 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4801 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     4861 actgtcgaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     4921 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagtaactc
     4981 gacatttcga ccgggccgcc agcacagtgg gggggggccc gcgattagtc caatttgtta
     5041 aagacaggat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
     5101 ctatagagta cgagccatag atagaataaa agattttatt tagtctccag aaaaaggggg
     5161 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaagccat tttgcaaggc
     5221 atggaaaaat acataactga gaatagagaa gttcagatca aggttaggaa cagagagaca
     5281 ggagaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg ctcagggcca
     5341 agaacagttg gaacagcaga atatgggcca aacaggatat ctgtggtaag cagttcctgc
     5401 cccggctcag ggccaagaac agatggtccc cagatgcggt cccgccctca gcagtttcta
     5461 gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt
     5521 gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa
     5581 taaaagagcc cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg
     5641 gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc
     5701 ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc attctgcatt
     5761 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     5821 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     5881 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5941 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6001 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     6061 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     6121 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     6181 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     6241 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     6301 agtccaaccc ggtaagacac gacttatagc cactggcagc agccactggt aacaggatta
     6361 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     6421 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     6481 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     6541 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     6601 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     6661 caaaaaggat cttcacctag atccttttgc ggccggccgc aaatcaatct aaagtatata
     6721 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6781 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6841 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6901 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6961 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     7021 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     7081 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     7141 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     7201 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     7261 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7321 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     7381 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7441 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7501 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7561 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7621 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7681 gaaaaataaa caaatagggg ttccgcgcac atttc
//
