LOCUS       sequence-75603-e         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of RFA1, RFA2, and RFA3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   He Z, Wong JM, Maniar HS, Brill SJ, Ingles CJ
  TITLE     Assessing the requirements for nucleotide excision repair proteins 
            of Saccharomyces cerevisiae in an in vitro system.
  JOURNAL   J Biol Chem. 1996 Nov 8;271(45):28243-9.
  PUBMED    8910442
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1996-11-8"; volume: "271(45)"; pages: "28243-9"
COMMENT     Sequence Label: pJM126
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     464..483
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        464..482
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    483..507
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             522..544
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cttggaagtc atcaaatgtc attccatagc cagtggaatg atgaaacaac ctttggagag
       61 tgcatccaac aacaatgggc aatcattatt tgtcaaggat gataacgata catcttccgg
      121 ctccagtccg ttacaaagaa ttctagaatt ttgtaagaag caatgtgagg gcaaagacgc
      181 taattcattc gctgttccca ttccattgat ctcgcaatcc ttgaatttgg atgaaactac
      241 cgtcagaaac tgctgtacga ccttgactga ccagggtttt atctacccaa cttttgatga
      301 caataacttc tttgccctat gaaaatatat agattatttt tttagcattt cttagtattc
      361 gtttgtgtat ttctactagg taagtaccaa aatttgccgg aaatagagtg gaaaatggaa
      421 ccatcgaatt cctgcagccc gggggatctc gatcccgcga aattaatacg actcactata
      481 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
      541 gagatataca tatgagcagt gttcaacttt cgaggggcga ttttcatagc atcttcacca
      601 ataagcaaag gtacgataat cccaccggtg gcgtttatca agtttataac accaggaaat
      661 ctgatggggc taacagcaac agaaagaatt tgatcatgat ttccgatggt atttaccata
      721 tgaaggctct gttgagaaac caagctgcat ccaagttcca gtcaatggaa ctacaaaggg
      781 gtgatatcat tcgcgtgata attgcagaac ctgctattgt cagggaaaga aagaaatacg
      841 ttcttttagt agatgacttt gagttggtcc agtcgcgtgc tgatatggtc aaccaaacta
      901 gtactttttt ggataactat ttctcagagc atccaaatga aaccttaaaa gacgaa
//
