LOCUS       sequence-75604-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressed flag-tagged NRBP1 (nuclear receptor binding protein 1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Mahrour N, Redwine WB, Florens L, Swanson SK, Martin-Brown S, 
            Bradford WD, Staehling-Hampton K, Washburn MP, Conaway RC, Conaway 
            JW
  TITLE     Characterization of Cullin-box sequences that direct recruitment of 
            Cul2-Rbx1 and Cul5-Rbx2 modules to Elongin BC-based ubiquitin 
            ligases.
  JOURNAL   J Biol Chem. 2008 Mar 21;283(12):8005-13. Epub 2008 Jan 10.
  PUBMED    18187417
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-03-21"; volume: "283(12)"; pages: "8005-13. Epub
            2008 Jan 10"
COMMENT     Sequence Label: pcDNA5/FRT-Flag-NRBP1
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc atggattaca aggatgacga cgataagctc
      121 gagatgtcgg agggggagtc ccagacagta cttagcagtg gctcagaccc aaaggtagaa
      181 tcctcatctt cagctcctgg cctgacatca gtgtcacctc ctgtgacctc cacaacctca
      241 gctgcttccc cagaggaaga agaagaaagt gaagatgagt ctgagatttt ggaagagtcg
      301 ccctgtgggc gctggcagaa gaggcgagaa gaggtgaatc aacggaatgt accaggtatt
      361 gacagtgcat acctggccat ggatacagag gaaggtgtag aggttgtgtg gaatgaggta
      421 cagttctctg aacgcaagaa ctacaagctg caggaggaaa aggttcgtgc tgtgtttgat
      481 aatctgattc aattggagca tcttaacatt gttaagtttc acaaatattg ggctgacatt
      541 aaggagaaca aggccagggt catttttatc acagaataca tgtcatctgg gagtctgaag
      601 caatttctga agaagaccaa aaagaaccac aagacgatga atgaaaaggc atggaagcgt
      661 tggtgcacac aaatcctctc tgccctaagc tacctgcact cctgtgaccc ccccatcatc
      721 catgggaacc tgacctgtga caccatcttc atccagcaca acggactcat caagattggc
      781 tctgtggctc ctgacactat caacaatcat gtgaagactt gtcgagaaga gcagaagaat
      841 ctacacttct ttgcaccaga
//
