LOCUS       sequence-75605-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of flag-tagged human Narg2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-Flag-Narg2
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc atgccagact acaaggacga tgatgacaag
      121 ctcgacatga gctccaagat ggtcataagt gaaccaggac tgaattggga tatttccccc
      181 aaaaatggcc ttaagacatt tttctctcga gaaaattata aagatcattc catggctcca
      241 agtttaaaag aactacgtgt tttatccaac agacgtatag gagaaaattt gaatgcctca
      301 gcaagttctg tagaaaatga gccggcagtt agttcagcaa ctcaagtaaa ggaaaaagtt
      361 aaaaccacaa ttggaatggt tcttcttcca aaaccaagag ttccttatcc tcgtttctct
      421 cgtttctcac agagagagca gaggagttat gtggacttgt tggttaaata cgcaaagatt
      481 cctgcaaatt ccaaagctgt tggaataaat aaaaatgact acttgcagta cttggatatg
      541 aaaaaacatg tgaacgaaga agttactgag ttcctaaagt ttttgcagaa ttctgcaaag
      601 aaatgtgcgc aggattataa tatgctttct gatgatgccc gtctcttcac agagaaaatt
      661 ttaagagctt gcattgaaca agtgaaaaag tattcagaat tctatactct ccacgaggtc
      721 accagcttaa tgggattctt cccattcaga gtagagatgg gattaaagtt agaaaaaact
      781 cttctcgcat tgggcagtgt aaaatatgtg aaaacagtat ttccctcaat gcctataaag
      841 ttgcagctgt caaaggacga tatagctacc attgaaacgt cagaacaaac agctgaagct
      901 atgcattatg atattagtaa agatccaaat gcagagaagc ttg
//
