LOCUS       sequence-75608-e         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged human KIAA0947.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-Flag-KIAA0947
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             128..151
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc
      121 catgccagat tacaaggatg acgatgacaa gatgatgccg ggcgagaccc attcggcggc
      181 gcccgggacg gcggcggacc tgtcgcgatg tcagggctgc gcctctctgc agcagaattt
      241 gaatgaatat gttgaagcat taattacctt gaaacaaaaa attatcaata cagataattt
      301 gttaacagaa tatcagaaga aatgtgatga gctgcagttt gcaagaagag agaatagtaa
      361 tctgcatcac caagtggaag agatgcttca aaaaatttct cctctacaga aatgtcagga
      421 agaactggga tctttaaaag cagagctaga agagaaaaag agttctttaa agttgtatca
      481 ggatactcat caggaatatg ctcgtgtaaa ggaagaatgc ttgaagagtg atgctcagaa
      541 gaagaaacta gaagctaagg tgaagaagct gcaagaggct gctgtcaagc aaactcagga
      601 cttcaagcaa ctgagaaatg aaaagaaaat acttgaaaag gaatttaaga agacacagga
      661 aaggcttgac gaattttcta aacagaaaaa tgaaaaggag ttgagacata ttggaacaca
      721 aatttcaagt gattcatatg gaagcataga taaaagaaaa gtgaaactgc ttctgaagga
      781 actctggctc tgtgtaaaca caacacacag actacctggt gaaggcagca ggtgtgtccc
      841 agaaaaacct gccaaagcaa tcaccagctc cagagtgcct ggggaagatg gtacgctacc
      901 tccaacacag ggcagccctc tc
//
