LOCUS       sequence-75614-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged human EAF1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-Flag-EAF1
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             97..120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc atgccagact acaaggacga tgatgacaag
      121 ctcgagatga atgggaccgc aaacccgctg ctggaccgcg aggaacattg cctgaggctc
      181 ggggagagct tcgagaagcg gccgcgggcc tccttccaca ctattcgtta tgattttaaa
      241 ccagcatcta tagacacttc ctgtgaagga gagcttcaag ttggcaaagg agatgaagtc
      301 acaattacac ttccacatat ccctggatcc acaccaccca tgactgtgtt caaggggaac
      361 aaacggcctt accagaaaga ctgtgtgctt attattaatc atgacactgg tgaatttgtg
      421 ctggaaaaac tcagtagcag cattcaggtg aagaaaacaa gagctgaggg cagcagtaaa
      481 atccaggccc gaatggaaca gcagcccact cgtcctccac agacgtcaca gccaccacca
      541 cctccaccac ctatgccatt cagagctcca acgaagcctc cagttggacc caaaacttct
      601 cccttgaaag ataacccctc acctgaacct cagttggatg acatcaaaag agagctgagg
      661 gctgaagttg acattattga acaaatgagc agcagcagtg ggagcagctc ttcagactct
      721 gagagctctt cgggaagtga tgacgatagc tccagcagtg gaggcgagga caatggccca
      781 gcctctcctc cgcagccttc acaccagcag ccctacaaca gtaggcctgc cgttgccaat
      841 ggaaccagcc ggccacaagg aagcaaccag ctcatgaacg ccctcagaaa tgacttgcag
      901 ttgagtgagt ctggcagtga cagtgatgac tagagatcca ctagtccagt gtggtggaat
      961 tctgca
//
