LOCUS       sequence-75619-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged human Med26-NTD- K74A K75A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-F-Med26-NTD- K74A K75A
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(563..580)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    569..793
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcatg ccagactaca aggacgatga tgacaagctc
      121 gagatgacag cggctccggc gtctccgcag cagatcaggg accggctgct gcaggccatc
      181 gacccccaga gcaacatccg gaacatggtg gcggtgctgg aagtcatctc cagcctggag
      241 aaatacccta ttaccaaaga ggcacttgag gaaacacgac ttgggaagct catcaacgac
      301 gtccgcaaga aaaccaagaa cgaggagctc gccaagcggg ccgcggcgct gctgcggagc
      361 tggcagaagc tcatcgagcc ggcacaccag catgaggcgg cgctgcgggg gctggcgggg
      421 gccaccggct ctgccaacgg gggcgcacac aactgccggc cgtgaggatc cactagtcca
      481 gtgtggtgga attctgcaga tatccagcac agtggcggcc gctcgatcga gtctagaggg
      541 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
      601 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
      661 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
      721 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
      781 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
      841 gcgccctgta gcggcgcatt aagcgcggcg gg
//
