LOCUS       sequence-75621-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged human Med26 R61A K62A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-F-Med26-R61A K62A
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcatg ccagactaca aggacgatga tgacaagctc
      121 gacatgacag cggctccggc gtctccgcag cagatcaggg accggctgct gcaggccatc
      181 gacccccaga gcaacatccg gaacatggtg gcggtgctgg aagtcatctc cagcctggag
      241 aaatacccta ttaccaaaga ggcacttgag gaaacacgac ttgggaagct catcaacgac
      301 gtcgccgcga aaaccaagaa cgaggagctc gccaagcggg ccaagaagct gctgcggagc
      361 tggcagaagc tcatcgagcc ggcacaccag catgaggcgg cgctgcgggg gctggcgggg
      421 gccaccggct ctgccaacgg gggcgcacac aactgccggc cggaggtggg ggcggctggc
      481 ccacccagga gcatccatga cctgaagagc cgcaatgacc tccagaggct gcccgggcag
      541 cggctggaca ggctgggcag ccgcaagcgc cggggtgacc agcgtgacct cggccaccca
      601 gggccgccac ccaaggtctc caaagctagc cacgaccccc tggtccccaa ctcatccccc
      661 ctccccacca acgggatcag tgggagtcca gagagcttcg ccagctccct ggatggcagt
      721 gggcatgcag gcccagaggg cagccgcctg gagcgtgacg agaatgacaa gcacagtggc
      781 aagatccccg tcaacgccgt gcgaccgcac accagctccc cgggcctggg caagccccct
      841 ggaccctgct tgcagccaaa ggcttcggtg ctgcagcagc tggacagggt ggacga
//
