LOCUS       sequence-75622-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of luciferase driven by mPer2 promoter fragment 
            containing mutated E-box2 (bases -112 to +98 with respect to 
            transcription start site at +1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Yoo SH, Ko CH, Lowrey PL, Buhr ED, Song EJ, Chang S, Yoo OJ, 
            Yamazaki S, Lee C, Takahashi JS
  TITLE     A noncanonical E-box enhancer drives mouse Period2 circadian 
            oscillations in vivo.
  JOURNAL   Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2608-13. Epub 2005 Feb 
            7.
  PUBMED    15699353
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2005-02-15"; volume: "102(7)"; pages: 
            "2608-13. Epub 2005 Feb 7"
COMMENT     Sequence Label: pGL3 basic Mut E2
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      325..334
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(397..415)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttctctatc gataggtacc gagctcttac gcgtgctagc cccgcggatc cgagcgtagc
       61 tctcaggttc cgccccgcca gtatgcaaat gaggtggcac tccgaccaat ggcgcgcgca
      121 ggggcgggct cagcgcgcgc ggtgctagtt tccactatgt gacagcggag ggcgacgcgg
      181 cggcagcggc gctactggga ctagcggctc cgggcggctg cggcgcaggc cgagcgcacc
      241 aagtgacggg ccgagcaagg gatctagagc agggctcgag atctgcgatc taagtaagct
      301 tggcattccg gtactgttgg taaagccacc atggaagacg ccaaaaacat aaagaaaggc
      361 ccggcgccat tctatccgct ggaagatgga accgctggag agcaactgca taaggctatg
      421 aagagatacg ccctggttcc tggaacaatt gcttttacag atgcacatat cgaggtggac
      481 atcacttacg ctgagtactt cgaaatgtcc gttcggttgg cagaagctat gaaacgatat
      541 gggctgaata caaatcacag aatcgtcgta tgcagtgaaa actctcttca attctttatg
      601 ccggtgttgg gcgcgttatt tatcggagtt gcagttgcgc ccgcgaacga catttataat
      661 gaacgtgaat tgctcaacag tatgggcatt tcgcagccta ccgtggtgtt cgtttccaaa
      721 aaggggttgc aaaaaatttt gaacgtgcaa aaaaagctcc caatcatcca aaaaattatt
      781 atcatggatt ctaaaacgga ttaccaggga tttcagtcga tgtacacgtt cgtcacatct
      841 catctacctc ccggttttaa tgaatacgat tttgtgccag agtccttcga tagggacaag
      901 acaattgcac tgatcatgaa ctcctctgga tctactggtc tgcctaaa
//
