LOCUS       sequence-75623-e         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of flag-tagged Med26-NTD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Takahashi H, Parmely TJ, Sato S, Tomomori-Sato C, Banks CA, Kong SE,
            Szutorisz H, Swanson SK, Martin-Brown S, Washburn MP, Florens L, 
            Seidel CW, Lin C, Smith ER, Shilatifard A, Conaway RC, Conaway JW
  TITLE     Human mediator subunit MED26 functions as a docking site for 
            transcription elongation factors.
  JOURNAL   Cell. 2011 Jul 8;146(1):92-104.
  PUBMED    21729782
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-07-8"; volume: "146(1)"; pages: "92-104"
COMMENT     Sequence Label: pcDNA5-F-Med26-NTD
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             94..117
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(515..532)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    521..745
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcatg ccagactaca aggacgatga tgacaagctc
      121 gacatgacag cggctccggc gtctccgcag cagatcaggg accggctgct gcaggccatc
      181 gacccccaga gcaacatccg gaacatggtg gcggtgctgg aagtcatctc cagcctggag
      241 aaatacccta ttaccaaaga ggcacttgag gaaacacgac ttgggaagct catcaacgac
      301 gtccgcaaga aaaccaagaa cgaggagctc gccaagcggg ccaagaagct gctgcggagc
      361 tggcagaagc tcatcgagcc ggcacaccag catgaggcgg cgctgcgggg gctggcgggg
      421 gccaccggct ctgccaacgg gggcgcacac aactgccggc cgtgaggatc cggtaccgtc
      481 gagtctagag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
      541 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
      601 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
      661 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
      721 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
      781 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      841 agcgtgaccg ctacacttgc cagcgcccta
//
